Feb 03, 2009  Best Answer: It lets you get an accurate plate count and therefore determine concentration of bacteria per mL. As the dilutions progress, you will reach a point where a reasonable number of colonies can be counted and multiplied by the dilution factor to get concentration. Disadvantages- does not separate. Direct dilution is the process by which Echo Liquid Handlers generates the IC 50 curve, transferring precise droplets of solution directly to individual assay wells, without tips. This straightforward process can generate a 12-point IC 50 curve from a stock solution and two intermediate dilutions, improving reliability and data quality.

Each calibration standard solution is prepared based on the previous calibration standard. The process involves taking a portion of the previous standard and diluting it with the solvent to obtain the next calibration standard. The errors introduced with each successive dilution drops proportionately with the solution concentration. Preparing a series of calibration standards by this method reduces the amount of required time. Most calibration standards span a large range of concentrations, so the accuracy of the calibration standard prepared increases.

Calibrations Solutions More Evenly Spaced. The dilution factor chosen for the series of calibration standards is achievable by using serial dilution.

Naan ee tamil movie online. The progression of calibration standard concentration is always a geometric series. Consider the example of making the first standard at 1/3 the concentration of the known, the next calibrant would be 1/9th the concentration of the known and the following two calibrants formed are 1/27th and 1/81st. This becomes a much greater advantage when the span of the calibration standards must cover several orders of magnitude in concentration.

Dilution is the act of mixing a chemical with other substance, usually distilled water to make it lighter in composition. It is usually done if the chemical concentration is too high than the desired composition. Thus, serial dilution simply means a series of repeated dilution performed on the same chemical basically to change its concentration. After performing the dilution, we need to know how much difference are the diluted chemical and the initial, undiluted ones. One way is by obtaining a factor called the Dilution Factor (DF).

The factor can be obtained by dividing the actual volume of the initial chemical used to the final volume of the chemical after water is added. As example, if 1.0 ml of 3M HCl is added with 9 ml of distilled water, the dilution factor becomes 1/10. In serial dilution, the entire dilution factor will be multiple together to be the actual dilution factor. There are several benefits of performing serial dilution. Serial dilution comes in handy when the solution is too concentrated to be used in experiments or ingredients preparation.

This is to ensure that the exact concentration can be obtained for the experiment to become success. Other than that, the diluted solution from this serial dilution can be used to count the concentration of the actual solution. By knowing the dilution factor of certain solution, the calculations of the concentration become easier and systematic. This method is applicable in several fields, not only in chemistry. Serial dilution is a simple yet efficient technique to determine the number of cells or organisms in a concentrated sample. First, take a portion of the sample and does serial dilution on it. Repeat the steps until the cells can be observed under the microscope when the diluted sample was observed.

Then, count the dilution factor and times it with the actual volume of the sample. Other than that, medicine administration require suitable dose for each patient with variable needs. This is where serial dilution is useful. References 1. Colin Ratledge, Bjorn Kristiansen, Basic Biotechnology, Second Edition, (2001) Cambridge University Press. Bonifacino, Marry Dasso, Joe B. Harford, Jennifer Lippincott-Schwartz and Kenneth M.

The full-page strip continued until 1971 when strip #1788 was not offered in full-page format—it was the last strip Foster drew. Download prince valiant comics rapidshare files. The first full page was strip #16, which appeared in the Sunday New Orleans Times Picayune. The internal dating changed from Saturday to Sunday with strip #66 (May 15, 1938).

Yamada, Short Protocols in Cell Biology: A Compendium of Methods from Current Protocols in Cell Biology, (2004), John Wiley and Sons, Inc., New Jersey. Aluizio Borem, Fabricio R.

Santos and David E. Bowen, Understanding Biotechnology, (2003), Pearson Education, Inc.

Campbell, Shawn O. Farrel, Biochemistry sixth edition (2009) Thomson Higher Education Corporation. For more information on managing or withdrawing consents and how we handle data, visit our Privacy Policy at: Show Details Necessary HubPages Device ID This is used to identify particular browsers or devices when the access the service, and is used for security reasons. Login This is necessary to sign in to the HubPages Service. Google Recaptcha This is used to prevent bots and spam. () Akismet This is used to detect comment spam.